As one of the most extensive and important protein post-translational modifications glycosylation plays a vital role in regulating organisms and is associated with various physiological and pathological processes. Recently researchers have focused on the need to characterize protein glycosylation sites structures and their degree of modification to better understand their biological functions while also looking for potential biomarkers for diagnosis and treatment of disease. Mass spectrometry (MS) is one of the most powerful tools used to study biomolecules including glycoproteins and glycans. With the continuous development of glycoproteomics and glycomics based on MS analysis more techniques have evolved and contribute to understanding the structure and function of glycoproteins and glycans. This book reviews advancements achieved in MS-based glycoproteomic analysis including a wide range of analytical methodologies and strategies involved in selective enrichment; as well as qualitative quantitative and data analysis together with their clinical applications. Significant examples are discussed to illustrate the principles laboratory protocols and advice for key implementation to ensure successful results. Mass Spectrometry–Based Glycoproteomics and Its Clinic Application will serve as a valuable resource to elucidate new techniques and their applications for students postdocs and researchers working in proteomics glycoscience analytical chemistry biochemistry and clinical medicine. Editor: Haojie Lu is a professor at Fudan University specializing in proteomics based on mass spectrometry with particular emphasis on novel technologies for separation and identification of low-abundant proteins and post-translationally modified proteins (including glycosylation) as well as relative and absolute quantification methods for proteomics.|Mass Spectrometry–Based Glycoproteomics and Its Clinic Application | Chemistry
