Novel Developments In Cryo‐Em Of Biological Molecules Resolution In Time And State Space | Science Client Lines

Cryo-EM as it is currently practiced in many laboratories is limited to the visualization of molecules that are in thermal equilibrium at the time before freezing. A further limitation is that the existing software does not fully exploit the information that is contained in the images of large ensembles of molecules in thermal equilibrium. This book is a collection of recent articles by the author reprinted with introductions and they mainly describe two novel methods in cryo-EM one computational and the other experimental that requires the use of a microfluidic device. Both methods have the capacity to shed light on the dynamic behavior of biomolecules. Combined they greatly expand the range of applications of cryo-EM. The book describes a successful approach in which based on cryo-EM data all states visited by the molecule in thermal equilibrium are mapped by manifold embedding-a method of geometric machine learning-and the energy landscape of the molecule is derived. It also discusses methods and biological results of time-resolved cryo-EM following a reaction in a non-equilibrium system over a short period of time and resulting in the capture of short-lived states that have been inaccessible by standard methods of cryo-EM. |Novel Developments in Cryo‐EM of Biological Molecules Resolution in Time and State Space | Science Client Lines